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Unité Matériaux et Transformations
CNRS UMR 8207 - Université de Lille

Les séminaires de l'UMET

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Séminaire UMET
Mercredi 11 Juin 2025 14:30 - Amphithéâtre Loison (ENSCL)
Chloé Grazon
. Institut des Sciences Moléculaires, University Bordeaux, CNRS, Bordeaux INP, UMR 5255, F-33400 Talence, France
  From Quantum Dots to Fluorescent Organic Nanoparticles: bright nanotools for biosensing

The in situ and real-time detection of analytes in complex biological media demands robust, sensitive, and stable biosensors capable of signal amplification. Luminescent nanoparticles (LNPs) are promising candidates, offering exceptional brightness and photostability compared to traditional dyes.1 These LNPs fall into two main categories: intrinsically luminescent, such as Quantum Dots (QDs), or doped NPs, where dyes are encapsulated within a matrix. For imaging and sensing applications, LNPs aim to achieve excellent brightness, enhanced photostability, and strong colloidal stability in water, outperforming conventional organic dyes. Classical FRET nanosensors typically involve a donor LNP conjugated with bioreceptors that bind to a ligand labeled with an acceptor dye. While bioreceptors optimization has advanced detection limits and dynamic ranges, the roles of dye type and spatial configuration in these systems remained underexplored. In this talk, we will compare organic fluorophores (e.g., Cy5, Texas Red) and QDs as FRET donors or acceptors, identifying key molecular parameters that enhance sensor performance to provide guidelines for FRET-based assays and diagnostics.2-3 Additionally, Fluorescent Organic Nanoparticles (dFONs) will be introduced as metal-free alternatives to QDs, with comparable brightness per volume. Obtained via nanoprecipitation of hydrophobic dyes, dFONs remain underutilized as biosensors due to limited functionalization strategies.4 We demonstrate an innovative maleimide-thiol surface functionalization approach, enabling applications such as intracellular thiol sensing in the µM range5 and biotinylation for biomarker development. These advancements position dFONs as versatile, ultra-bright, and metal-free tools for next-generation diagnostics.

 

References : 

1. A. Ashoka, et al., Chem. Soc. Rev 2023, DOI : 10.1039/D2CS00464J

2. C. Grazon et al., Chemical Science 2022, DOI : 10.1039/D1SC06921G

3. C. Grazon, R. Baer et al., Nature Communications 2020, DOI : 10.1038/s41467-020-14942-5

4. J. Daniel et al., CRAS 2024, DOI : 10.5802/crchim.294

5. O. Dal Pra et al., Small Methods, DOI : 10.1002/smtd.202400716

Séminaire UMET
Mardi 1 Avril 2025 10:30 - Grande Salle de Réunion, Institut Chevreul
Mérope Chardelin
Géosciences Montpellier
  Progressive strain localization and fluid-assisted deformation in mantle exhumation during rifting : petrostructural constraints from the Zabargad peridotites, Red Sea.
Séminaire UMET
Jeudi 20 Mars 2025 10:30 - Bâtiment Chevreul
Professeur Antoine Debuigne
CERN, Liège
  "Advances in Reversible Deactivation Radical Polymerization and Emulsion-Templating Polymerization for Drug Delivery and Tissue Engineering Applications."
Séminaire UMET
Lundi 17 Mars 2025 10:30 - Amphithéâtre bâtiment Chevreul
Gregor Golabek
Bayerisches Geoinstitut, Université de Bayreuth, Bayreuth, Allemagne
  Outgassing and atmosphere formation on terrestrial bodies
Séminaire UMET
Jeudi 16 Janvier 2025 10:30 - Grande Salle de Réunion, Institut Chevreul
Agnès Fienga et Clément Ganino
Laboratoire GéoAzur, Université Côte d'Azur
  L’intérieur des planètes telluriques révélé par les déformations de marées
Séminaire UMET
Jeudi 9 Janvier 2025 10:30 - Salle 202, Bâtiment C6
Valentine Megevand
Muséum National d'Histoire Naturelle
  Tardi-magmatism or alteration: What do the iddingsite veins in the NWA 817 Nakhlite tell us about the Amazonian history of Mars?
 
UMET - Unité Matériaux et Transformations
CNRS UMR 8207
Université de Lille
Bâtiment C6
59655 Villeneuve d'Ascq
France
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