Laurent PACCOU

Engineer

Bâtiment P5 - 241
Unité Matériaux et Transformations
Cité scientifique, Bât. P5
Université de Lille
59655 Villeneuve d'Ascq
France
Tel: +33 (0)3 20 43 43 35/+33 (0)3 20 43 49 96
Fax: +33 (0)3 20 43 40 84
Email : Send an email to Laurent PACCOU
ORCID Identifier :

Research team: Matériaux moléculaires et thérapeutiques

Warning: getimagesize(..//Instruments/ImagesDB/s_img-187-2064281652.png): failed to open stream: No such file or directory in /var/www/html/Private/Instruments/show.inc.php on line 47

microcalorimeter MicroDSC7 evo (Setaram)

Research team: Matériaux moléculaires et thérapeutiques.

Contacts: Alain HEDOUX, Laurent PACCOU.

the μDSC7 evo can meet the requirements of a wide range of applications, especially in the following areas:
- Life sciences - pharmaceutical: protein denaturation / aggregation in solution, powder or gel, solid state polymorphism, amorphism,
- Food: protein denaturation / aggregation, polysaccharide fusion / gelation, gelatin, starch, emulsion stability,
- Polymers: influence of pressure on glass transitions to supercritical conditions,
technical characteristics

• temperature range -45 ° C to 120 ° C
• programming speed from 0.001 to 2 ° C per min,
• Hastelloy cell volume up to 1 mL
• Use in isothermal mode (calorimeter) or temperature programming (DSC).
• Ability to study samples in all forms: liquid, gel, powder, solid.

 

Warning: getimagesize(..//Instruments/ImagesDB/s_img-186-2125491069.png): failed to open stream: No such file or directory in /var/www/html/Private/Instruments/show.inc.php on line 47

Raman µspectrometer Renishaw InVia , suitable for medium-frequency studies (150-1800 cm-1)

Research team: Matériaux moléculaires et thérapeutiques.

Contacts: Yannick GUINET, Alain HEDOUX, Laurent PACCOU.

Analysis of the dynamics of glass-forming molecular materials in their amorphous and crystalline states, mechanisms of phase transformation, and stabilization of amorphous states of materials of therapeutic interest (small molecules, proteins) by excipients

• lasers lmbda = 514 nm and 785 nm

Sample environment

• Platinum LinKam Scientific Instrument see part Heat treatment
• XYZ Platinum for cartography
• Anvil cell diamond Chervin type pressure generator 40 GPa
• CryoDAC-ST (Alamax Easy lab) cryoDAC-ST anvils cell pressure 15 GPa max and cryostat ST500b (Janis) temperature control from 3.5K to 475K.
• Humidity Controlled Platinum GenRH (Surface Measurement Systems)
• HR from 1 to 98% accuracy 0.8%

 

Warning: getimagesize(..//Instruments/ImagesDB/s_img-184-997738696.png): failed to open stream: No such file or directory in /var/www/html/Private/Instruments/show.inc.php on line 47

Raman Spectrometer XY Dilor, suitable for Low Frequency Macro Studies (5-500 cm-1)

Research team: Matériaux moléculaires et thérapeutiques.

Contacts: Alain HEDOUX, Yannick GUINET, Laurent PACCOU.

Analysis of the dynamics of glass-forming molecular materials in their amorphous and crystalline states, mechanisms of phase transformation, and stabilization of amorphous states of materials of therapeutic interest (small molecules, proteins) by excipients
Accessories

• Macro Output: Cryostat Optistat CF-V (Oxford Instrument) Temperature Control 500K to 4.2K
• Macro output: Cryostream 800 (Oxford Instrument) temperature control 500K to 80K with dry nitrogen flow.
• Micro Output: Microstat He (Oxford Instrument) temperature control 500K to 5K

 

Instruments in charge

Latest scientific contributions in LillOA (Lille Open Archive)

Publications

21. M. Khalaji, M.K. Dudek, L. Paccou, F. Danede, Y. Guinet, A. Hedoux, Description of an original molecular ordering process into a disordered crystalline form: the atypical low-temperature transformation of the disordered form III of linezolid, Physical Chemistry Chemical Physics 26;30, 20709-20716 (2024), [doi: 10.1039/D4CP02104E, LillOA]
20. Y. Guinet, L. Paccou, A. Hedoux, Low-Frequency Raman Spectroscopy: An Exceptional Tool for Exploring Metastability Driven States Induced by Dehydration, Pharmaceutics 15;7, 1955 (2023), [doi: 10.3390/pharmaceutics15071955, LillOA]
19. Y. Guinet, L. Paccou, A. Hedoux, Co-Amorphous Versus Deep Eutectic Solvents Formulations for Transdermal Administration, Pharmaceutics 15;10, 1710 (2023), [doi: 10.3390/pharmaceutics15061710, LillOA]
18. T. Rongthong, A. Qnouch, M. Maue Gehrke, L. Paccou, P. Oliveira, F. Danede, J. Verin, C. Vincent, J.-F. Willart, F. Siepmann, J. Siepmann, Silicone matrices for controlled dexamethasone release: toward a better understanding of the underlying mass transport mechanisms., Regenerative Biomaterials 10, rbad008 (2023), [doi: 10.1093/rb/rbad008, LillOA]
17. Y. Guinet, L. Paccou, A. Hedoux, Analysis of Co-Crystallization Mechanism of Theophylline and Citric Acid from Raman Investigations in Pseudo Polymorphic Forms Obtained by Different Synthesis Methods, Molecules 28;4, 1605 (2023), [doi: 10.3390/molecules28041605, LillOA]
16. Y. Guinet, L. Paccou, A. Hedoux, Mechanism for Stabilizing an Amorphous Drug Using Amino Acids within Co-Amorphous Blends, Pharmaceutics 15;2, 337 (2023), [doi: 10.3390/pharmaceutics15020337, LillOA]
15. B. Moutamenni, N. Tabary, A. Mussi, J. Dhainaut, C. Ciotonea, A. Fadel, L. Paccou, Y. Guinet, A. Hedoux, Milling-Assisted Loading of Drugs into Mesoporous Silica Carriers: A Green and Simple Method for Obtaining Tunable Customized Drug Delivery, Pharmaceutics 15;2, 390 (2023), [doi: 10.3390/pharmaceutics15020390, LillOA]
14. T. Rongthong, A. Qnouch, M.M. Gehrke, F. Danede, J.-F. Willart, P.F.M. De Oliveira, L. Paccou, G. Tourrel, P. Stahl, J. Verin, P. Toulemonde, C. Vincent, F. Siepmann, J. Siepmann, Long term behavior of dexamethasone-loaded cochlear implants: In vitro & in vivo, International Journal of Pharmaceutics: X 4, 100141 (2022), [doi: 10.1016/j.ijpx.2022.100141, LillOA]
13. Y. Guinet, L. Paccou, F. Danede, P. Derollez, A. Hedoux, Identification of incommensurability in L-leucine: can lattice instabilities be considered as general phenomena in hydrophobic amino acids?, Physical Chemistry Chemical Physics 24, 27023-27030 (2022), [doi: 10.1039/d2cp00989g, LillOA]
12. J.-F. willart, S. Ouerghemmi, N. Descamps, P. Heijboer, L. Paccou, F. Danede, M. Descamps, The melting of glucose, International Journal of Pharmaceutics 626, 122185 (2022), [doi: 10.1016/j.ijpharm.2022.122185, LillOA]
11. Y. Guinet, L. Paccou, F. Danede, A. Hedoux, Revisiting the phase transition sequence in L-methionine: Description of the disordering mechanism in an essential amino acid, Journal of Chemical Physics 156;3, 034501 (2022), [doi: 10.1063/5.0077743, LillOA]
10. B. Moutamenni, N. Tabary, L. Paccou, Y. Guinet, A. Hedoux, MAL: High performance method for loading hydrophobic molecular materials into MCM-41 mesoporous silica – Analysis of confined L-tryptophan by Raman spectroscopy, Journal of Molecular Structure 1254, 132383 (2022), [doi: 10.1016/j.molstruc.2022.132383, LillOA]
9. N. Schaeffer, D.O. Abranches, L.P. Silva, M.A. Martins, P.J. Carvalho, O. Russina, A. Triolo, L. Paccou, Y. Guinet, A. Hedoux, J.A. Coutinho, Non-Ideality in Thymol + Menthol Type V Deep Eutectic Solvents, ACS Sustainable Chemistry & Engineering 9;5, 2203-2211 (2021), [doi: 10.1021/acssuschemeng.0c07874, LillOA]
8. Y. Guinet, L. Paccou, F. Danede, A. Hedoux, Confinement of molecular materials using a solid-state loading method: a route for exploring new physical states and their subsequent transformation highlighted by caffeine confined to SBA-15 pores, RSC Advances 11;55, 34564-34571 (2021), [doi: 10.1039/D1RA05757J, LillOA]
7. B. Malfait, L. Paccou, N. T. Correia, Y. Guinet, A. Hedoux, Identification of an amorphous-amorphous two-step transformation in indomethacin embedded within mesoporous silica, Microporous and Mesoporous Materials 328, 111502 (2021), [doi: 10.1016/j.micromeso.2021.111502, LillOA]
6. M. Guerain, Y. Guinet, N. T. Correia, L. Paccou, F. Danede, A. Hedoux, Polymorphism and stability of ibuprofen/nicotinamide cocrystal: The effect of the crystalline synthesis method, International Journal of Pharmaceutics 584, 119454 (2020), [doi: 10.1016/j.ijpharm.2020.119454, LillOA]
5. Y. Guinet, L. Paccou, A. Hedoux, A detailed description of the devitrification mechanism of D-mannitol, Physical Chemistry Chemical Physics 22, 5011-5017 (2020), [doi: 10.1039/c9cp05287a, LillOA]
4. Y. Guinet, L. Paccou, A. Hedoux, Analysis of xylitol – citric acid system forming deep eutectic solvent: Application for dissolving poorly water-soluble drugs. A combination of calorimetric and Raman investigations, Journal of Molecular Liquids 318, 114317 (2020), [doi: 10.1016/j.molliq.2020.114317, LillOA]
3. M. Latreche, J.-F. willart, L. Paccou, Y. Guinet, F. Danede, A. Hedoux, Contribution of low-frequency Raman spectroscopy to determine the solubility curves of drugs in polymers: The case of paracetamol/PVP, European Journal of Pharmaceutics and Biopharmaceutics 154, 222-227 (2020), [doi: 10.1016/j.ejpb.2020.07.012, LillOA]
2. A. Dupont, M. Guerain, F. Danede, L. Paccou, Y. Guinet, A. Hedoux, J.-F. willart, Kinetics and mechanism of polymorphic transformation of sorbitol under mechanical milling, International Journal of Pharmaceutics 590, 119902 (2020), [doi: 10.1016/j.ijpharm.2020.119902, LillOA]
1. T. Starciuc, B. Malfait, F. Danede, L. Paccou, Y. Guinet, N. T. Correia, A. Hedoux, Trehalose or sucrose; which of the two should be used for stabilizing proteins in the solid state? A dilemma investigated by in-situ micro-Raman and dielectric relaxation spectroscopies during and after freeze-drying, Journal of Pharmaceutical Sciences 109;1, 496-504 (2019), [doi: 10.1016/j.xphs.2019.10.055, LillOA]

Full list (The link could be slow)

Conference presentations

10. M. Guerain, P. Derollez, C. Dejoie, N. T. Correia, F. Danede, F. Affouard, L. Paccou, Y. Guinet, A. Hedoux, C. Dejoie, Analyse structurale de matériaux moléculaires thérapeutiques cocristallisés par diffraction des rayons X, Communication dans un congrès sans actes, Rayons X et Matière 2019, Nancy, France, Nov 2019 [LillOA]
9. M. Gehrke, T. Rongthong, G. Tourrel, D. Gnansia, P. Oliveira, J.-F. Willart, L. Paccou, Y. Guinet, A. Hedoux, C. Vincent, F. Siepmann, J. Siepmann, Homogeneity, drug release and swelling of dexamethasone loaded cochlear implants, Poster, 11th world meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical technology, Granada, Espagne, Mar 2018 [LillOA]
8. B. Malfait, N. T. Correia, A. Mussi, L. Paccou, Y. Guinet, A. Hedoux, Solid-state loading of pharmaceutical materials within mesoporous silica matrices, Communication dans un congrès sans actes, 1st International Conference on Contemporary Pharmacy Challenges: 1st International Conference on Contemporary Pharmacy Challenges:, Wisla, Pologne, Sep 2018 [LillOA]
7. T. Starciuc, L. Paccou, Y. Guinet, A. Hedoux, Mechanism of protein stabilization analyzed from in-situ Raman investigations during freeze-drying procedures, Conférence invitée, Freeze-drying of pharmaceuticals & biologicals, Garmisch-Partenkirchen, Allemagne, Sep 2018 [LillOA]
6. T. Starciuc, A. Hedoux, Y. Guinet, L. Paccou, Mechanisms of protein denaturation and stabilization analyzed in-situ during the freeze-drying process by micro-Raman spectroscopy, Conférence invitée, 8th International Discussion Meeting on Relaxations in Complex Systems (8IDMRCS), Wisla, Pologne, Jul 2017 [LillOA]
5. G. Bellavia, L. Paccou, Y. Guinet, A. Hedoux, The solvent dynamical transition is a necessary precursor to promote the protein unfolding, Communication dans un congrès sans actes, International Symposium On the Properties of Water, ISOPOW XIII, Lausanne, Suisse, Jun 2016 [LillOA]
4. T. Starciuc, L. Paccou, Y. Guinet, A. Hedoux, Protein denaturation and stabilization during freeze-drying analyzed from in-situ micro Raman investigations, Conférence invitée, International Symposium On the Properties of Water, ISOPOW XIII, Lausanne, Suisse, Jun 2016 [LillOA]
3. G. Bellavia, L. Paccou, Y. Guinet, A. Hedoux, Role of glycerol on trehalose to enhance biostability in relation with fast anharmonic and quasi-harmonic motions, Communication dans un congrès sans actes, ProtStab2014 : 10th International Conference on Protein Stabilisation, Stresa, Italie, May 2014 [LillOA]
2. A. Hedoux, L. Paccou, Y. Guinet, The contribution of the low-frequency Raman spectroscopy to the structural description of disordered molecular systems and their transformations: application to pharmaceuticals, Conférence invitée, SciX 2015, Providence (Rhode Island), Etats-Unis d'Amérique, Oct 2015 [LillOA]
1. A. Hedoux, L. Paccou, Y. Guinet, Solid state transformations of APIs induced by various stresses analyzed in real time by Raman spectroscopy, Conférence invitée, AMPTEC, Lille, France, Jul 2014 [LillOA]

Full list (The link could be slow)